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Oral Health and Preventive Dentistry
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Oral Health Prev Dent 18 (2020), Open Access     12. Feb. 2020
Oral Health Prev Dent 18 (2020), Open Access  (12.02.2020)

Open Access ORAL MEDICINE, Online Article, Page 311-318, doi:10.3290/j.ohpd.a43365, PubMed:32618454


Online Article: Effect of a Single Application of CPP-ACPF Varnish on the Prevention of Erosive Tooth Wear: An AAS, AFM and SMH Study
Gokkaya, Berna / Ozbek, Nil / Guler, Zeliha / Akman, Suleyman / Sarac, A. Sezai / Kargul, Betul
Purpose: The aim of this study was to investigate the in vitro effect of casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) and fluoride-containing varnish on prevention enamel erosive tooth wear.
Materials and Methods: A total of 28 enamel samples were prepared from human molars, divided into four groups: CPP-ACPF varnish, TCP-F varnish, NaF varnish, and deionised water. For the remineralisation process stimulated human pooled saliva was used. After treatment, all enamel samples were exposed to 10 ml of Coca Cola. Ca++ release was determined by atomic absorption spectroscopy (AAS). The surface topography was evaluated by atomic force microscopy (AFM). Surface microhardness of enamel was analysed and SMHR % (surface microhardness reduction ) was calculated. Data were analysed with repeated measures analysis of variance (ANOVA).
Results: Deionised water demonstrated a statistically significantly higher Ca+2 release compared to those of groups NaF > fTCP > CPP-ACPF, respectively (p <0.01). All groups measured for root-mean-square-roughness (Rrms) showed a statistically significantly difference of 6 × 6 μm2 and 12 × 12 μm2 enamel area (p <0.05) compared with a negative control group. CPP-ACPF varnish showed rougher surfaces than all remineralisation groups. SMHR % of enamels were as follows: CPP-ACPF < fTCP < NaF < deionised water (p <0.01).
Conclusion: According to the findings of this study; CPP-ACP containing agents have a statistically statistically significant effect on preventing dental erosion. Among these, CPP-ACPF-containing remineralisation agents have the most effect on the remineralisation process.

Keywords: AFM, AAS, CPP-ACPF, erosion, SMH