Wir verwenden Cookies, um für diese Website Funktionen wie z.B. Login, einen Warenkorb oder die Sprachwahl zu ermöglichen. Weiterhin nutzen wir für anonymisierte, statistische Auswertungen der Nutzung Google Analytics, welches Cookies setzt. Mehr Informationen finden Sie in unserer Datenschutzerklärung.
OK, ich bin einverstanden Ich möchte keine Google Analytics-Cookies
Oral Health and Preventive Dentistry
Login:
Benutzername:

Kennwort:

Plattform:

Kennwort vergessen?

Registrieren

Oral Health Prev Dent 17 (2019), Nr. 4     16. Aug. 2019
Oral Health Prev Dent 17 (2019), Nr. 4  (16.08.2019)

Seite 375-383, doi:10.3290/j.ohpd.a42684, PubMed:31204391, Sprache: Englisch


In Vitro Effects of Arginine-Containing Toothpastes on Cariogenic Biofilms
Berto, Luciana Aranha / Lauener, Anic / Carvalho, Thiago Saads / Lussi, Adrian / Eick, Sigrun
Purpose: The effects of arginine as a toothpaste additive were assessed on oral streptococci with and without a known arginine deiminase system (ADS) and cariogenic biofilms.
Materials and Methods: Suspensions of Streptococcus mutans, S. sobrinus and the ADS-positive (ADS+) S. sanguinis and S. gordonii were cultured with or without 1.5% L-arginine for 24 h. Thereafter, biofilms consisting of the four species were formed on polystyrene surfaces with or without 1.5% L-arginine for up to 10 d. Finally, biofilms that formed on enamel surfaces were exposed to a daily mechanical cleaning with an arginine and sodium monofluorophosphate (SMF+Arg)-containing toothpaste, a sodium monofluorophosphate fluoride (SMF)-containing toothpaste or a negative control for up to 10 weeks. At different incubation times, the pH in the culture media, the citrulline production and the percent of ADS+ bacteria within the biofilms were determined. Microsurface hardness loss was quantified in the experiments using enamel specimens.
Results: In the presence of 1.5% arginine, S. sanguinis and S. gordonii showed a high level of production of citrulline after 6 h of incubation, together with an increase in the pH when compared to S. mutans and S. sobrinus. With arginine supplementation, the percentage of ADS+ species was higher at 1, 2 and 4 days and citrulline production was higher at all days of biofilm formation on polystyrene surfaces. After 4 and 10 weeks of treating biofilms on enamel surfaces, the SMF+Arg group had a higher proportion of ADS+ strains than the SMF group; at 4 weeks, the pH was higher in the SMF+Arg group. Loss of enamel hardness was the lowest in the SMF+Arg group and was significantly less in the SMF+Arg group than in the control group after 2, 4 and 10 weeks of treatment.
Conclusion: Toothbrushing using an arginine-containing toothpaste may protect against dental caries.

Schlagwörter: arginine, citrulline, multispecies biofilm, microsurface hardness, oral streptococci
Volltext (keine Berechtigung) einzeln als PDF kaufen (20.00 €)Endnote-Export