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Oral Health & Preventive Dentistry
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Oral Health Prev Dent 15 (2017), No. 1     23. Feb. 2017
Oral Health Prev Dent 15 (2017), No. 1  (23.02.2017)

Page 73-77, doi:10.3290/j.ohpd.a37716, PubMed:28232977


Chemical Methods for Cleaning Conventional Dentures: What is the Best Antimicrobial Option? An In Vitro Study
Pires, Carine W. / Fraga, Sara / Beck, Aline C. O. / Braun, Kátia O. / Peres, Paulo E. C.
Purpose: To investigate the antimicrobial efficacy of different chemical agents used for denture cleaning.
Materials and Methods: Biofilm samples collected from 10 removable dentures were subjected to 10 disinfection protocols: distilled water for 30 min (negative control); 1% sodium hypochlorite for 10 min (positive control); diluted sodium hypochlorite for 10 min; vinegar for 20 min; 0.2% peracetic acid for 5 min; alkaline peroxide solution for 5 min; alkaline peroxide solution for 30 min; 0.12% chlorhexidine digluconate for 10 min; 0.05% sodium salicylate solution for 10 min; and enzymatic detergent for 2 min. Each of the samples was plated on petri dishes with Mueller-Hinton agar. The numbers of microbial colonies after 48 h at 37°C were compared using the Kruskal-Wallis and Dunn's tests (α = 0.05).
Results: Diluted sodium hypochlorite, vinegar, and chlorhexidine digluconate inhibited bacterial growth, with an effect similar to that of 1% sodium hypochlorite. The 0.2% peracetic acid and 0.05% sodium salicylate solutions were ineffective against bacterial growth, while enzymatic detergent and alkaline peroxide achieved an intermediate effect.
Conclusion: Diluted sodium hypochlorite, vinegar, and chlorhexidine digluconate can be considered adequate products for cleaning dentures due to their potential for inhibiting bacterial growth, similar to 1% sodium hypochlorite. However, the effect of these chemical agents on acrylic resin (polymethylmethacrylate) denture base materials needs to be examined to provide complete information about their clinical indication.

Keywords: chemical cleaning, denture hygiene, microbiology, oral health