Oral Health Prev Dent 13 (2015), No. 4 1. Sep. 2015
Oral Health Prev Dent 13 (2015), No. 4 (01.09.2015)
Page 341-348, doi:10.3290/j.ohpd.a30601, PubMed:24046822
Antimicrobial Effect of Jasminum grandiflorum L. and Hibiscus rosa-sinensis L. Extracts Against Pathogenic Oral Microorganisms - An In Vitro Comparative Study
Nagarajappa, Ramesh / Batra, Mehak / Sharda, Archana J. / Asawa, Kailash / Sanadhya, Sudhanshu / Daryani, Hemasha / Ramesh, Gayathri
Purpose: To assess and compare the antimicrobial potential and determine the minimum inhibitory concentration (MIC) of Jasminum grandiflorum and Hibiscus rosa-sinensis extracts as potential anti-pathogenic agents in dental caries.
Materials and Methods: Aqueous and ethanol (cold and hot) extracts prepared from leaves of Jasminum grandiflorum and Hibiscus rosa-sinensis were screened for in vitro antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus using the agar well diffusion method. The lowest concentration of every extract considered as the minimum inhibitory concentration (MIC) was determined for both test organisms. Statistical analysis was performed with one-way analysis of variance (ANOVA).
Results: At lower concentrations, hot ethanol Jasminum grandiflorum (10 μg/ml) and Hibiscus rosa-sinensis (25 μg/ml) extracts were found to have statistically significant (P ≤ 0.05) antimicrobial activity against S. mutans and L. acidophilus with MIC values of 6.25 μg/ml and 25 μg/ml, respectively. A proportional increase in their antimicrobial activity (zone of inhibition) was observed.
Conclusion: Both extracts were found to be antimicrobially active and contain compounds with therapeutic potential. Nevertheless, clinical trials on the effect of these plants are essential before advocating large-scale therapy.
Keywords: agar well diffusion, antimicrobial activity, dental caries, Lactobacillus acidophilus, Streptococcus mutans